Review

mouse myeloma cell line s194 5xx0 (ATCC)

ATCC is a verified supplier

ATCC manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

ATCC mouse myeloma cell line s194 5xx0
Mouse Myeloma Cell Line S194 5xx0, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+myeloma+cells/S194%2F5%2EXXO%2EBU%2E1/pm42270621-327-10-16
Average 94 stars, based on 37 article reviews

mouse myeloma cell line s194 5xx0 - by Bioz Stars, 2026-09

94/100 stars

Images

Single Cell:

Article Title: Polynucleotides encoding antibodies that specifically bind to human IL-15
Article Snippet: .. Single cell suspensions of lymphocytes were fused to P3X63Ag8.653 non secreting mouse myeloma cells (ATCC, CRL-1580). .. Cells were plated at approximately 1×105 cells/mL in flat bottom microtiter plates, followed by 2 week incubation in selective medium containing, besides usual reagents, 10% fetal clone serum and 1×HAT (Sigma).

Article Title: Antibodies to CD40 with enhanced agonist activity
Article Snippet: .. For example, single cell suspensions of splenic lymphocytes from immunized mice can be fused to Sp2/0 nonsecreting mouse myeloma cells (ATCC, CRL 1581) with 50% PEG. .. Cells are plated at approximately 2×10′ in flat bottom microtiter plate, followed by a two week incubation in selective medium containing 10% fetal Clone Serum, 18% “653” conditioned media, 5% origen (IGEN), 4 mM L-glutamine, 1 mM sodium pyruvate, 5 mM HEPES, 0.055 mM 2-mercaptoethanol, 50 units/ml penicillin, 50 mg/ml streptomycin, 50 mg/ml gentamycin and 1×HAT (Sigma).

Cell Culture:

Article Title: A monoclonal antibody-based electrochemical immunosensor for porcine epidemic diarrhea virus.
Article Snippet: .. Mouse myeloma cells (SP2/0, ATCC CRL-1581, Manassas, VA, USA), Vero E6 cells (ATCC CRL-1586, Manassas, VA, USA), and human embryonic kidney cells (HEK-293T, ATCC CRL-3261, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, SigmaAldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (P/S) at 37 °C with 5% CO2. .. The gene fragment encoding the S protein (residues 639–792) of the PEDV strain LJX01/ GS/2014 was amplified and cloned into the prokaryotic expression vector pET-30a(+) (Merck KGaA, Burlington, MA, USA).

Modification:

other:

Article Title: Polynucleotides encoding antibodies that specifically bind to human IL-15
Article Snippet: Cells were plated at approximately 1×105 cells/mL in flat bottom microtiter plates, followed by 2 week incubation in selective medium containing, besides usual reagents, 10% fetal clone serum and 1×HAT (Sigma).